Mrfp-gfp tandem fluorescent-tagged lc3 instruction

Mammalian expression of rat LC3 fused to mRFP and EGFP autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3. 17 Nov 2016 The GFP-LC3-RFP-LC3?G probe is a simple and quantitative method to Red fluorescent protein (RFP)-GFP tandem fluorescent-tagged LC3 for technical instruction regarding the ratiometric imaging analysis; and Dr. Protein, Tandem Fluorescent-Tagged LC3 | During the process of autophagy, marker protein, mRFP-GFP-LC3 tandem-tagged fluores- cent protein (tfLC3) 24 Oct 2018 Kimura and collaborators [45] showed that using a tandem-tagged mRFP-GFP-LC3, GFP fluorescence (pKa 5.9) is quenched in the acidic 31 May 2012 Tandem fluorescent-tagged LC3 (mRFP-EGFP-LC3) is a convenient assay for monitoring autophagic flux based on different pH stability of mRFP-GFP tandem fluorescent-tagged LC3 (tfLC3). Kenta Imai, Junya Hasegawa, Tamotsu Yoshimori. METHOD. 1. Coat coverslips with 0.1 mg/ml collagen 4 Sep 2018 Tandem fluorescent-tagged LC3 (mRFP-EGFP-LC3) is a convenient infection was performed according to the manufacturer's instructions. Using the RFP-GFP/LC3 stable cell line, we found that even upon syntaxin-5 .. using Lipofectamine 2000, according to the manufacturer's instructions (Invitrogen). .. process by a novel reporter protein, tandem fluorescent-tagged LC3.